![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2557031
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-09)
miniCMV promoter-Anti-GFP-mnotch-TEV protease-NLS
The Notch receptor is a natural cell surface receptor whose extramembrane domain can be replaced by a variety of receptors, and the intramembrane domain can be replaced with a variety of transcription factors to construct a customized cell signaling pathway. We retained the conserved mmotch transmembrane domain, replacing the extracellular domain with anti-GFP and the intracellular domain with the TEV protease, thereby converting the extracellular signal into an intracellular signal.We inserted the miniCMV promoter upstream so that the components we constructed were expressed at low levels in the cell.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 455
Illegal PstI site found at 499
Illegal PstI site found at 818 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
Illegal PstI site found at 455
Illegal PstI site found at 499
Illegal PstI site found at 818
Illegal NotI site found at 1616 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2109
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 455
Illegal PstI site found at 499
Illegal PstI site found at 818 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 455
Illegal PstI site found at 499
Illegal PstI site found at 818
Illegal NgoMIV site found at 254
Illegal NgoMIV site found at 959
Illegal NgoMIV site found at 1136
Illegal NgoMIV site found at 1674
Illegal AgeI site found at 71 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1295
[edit]
Categories
Parameters
//chassis/eukaryote
None |