Composite

Part:BBa_K2557031

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)
Revision as of 12:54, 9 October 2018 by Registry (Talk | contribs)

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miniCMV promoter-Anti-GFP-mnotch-TEV protease-NLS

The Notch receptor is a natural cell surface receptor whose extramembrane domain can be replaced by a variety of receptors, and the intramembrane domain can be replaced with a variety of transcription factors to construct a customized cell signaling pathway. We retained the conserved mmotch transmembrane domain, replacing the extracellular domain with anti-GFP and the intracellular domain with the TEV protease, thereby converting the extracellular signal into an intracellular signal.We inserted the miniCMV promoter upstream so that the components we constructed were expressed at low levels in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 455
    Illegal PstI site found at 499
    Illegal PstI site found at 818
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
    Illegal PstI site found at 455
    Illegal PstI site found at 499
    Illegal PstI site found at 818
    Illegal NotI site found at 1616
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2109
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 455
    Illegal PstI site found at 499
    Illegal PstI site found at 818
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 455
    Illegal PstI site found at 499
    Illegal PstI site found at 818
    Illegal NgoMIV site found at 254
    Illegal NgoMIV site found at 959
    Illegal NgoMIV site found at 1136
    Illegal NgoMIV site found at 1674
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1295


[edit]
Categories
//chassis/eukaryote
Parameters
None