Regulatory
Part:BBa_K2610002
Designed by: Charlotte de Ceuninck van Capelle Group: iGEM18_Leiden (2018-10-09)
Revision as of 12:05, 9 October 2018 by ChielvanAmstel (Talk | contribs)
pIbpA
Usage and Biology
Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)
Figure 1 caption
<img src="" max-width="30%">
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |