Coding

Part:BBa_K2690001:Design

Designed by: David Doyle   Group: iGEM18_SUIS_Shanghai   (2018-10-09)
Revision as of 11:47, 9 October 2018 by Davystar (Talk | contribs)

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PvsA - Vibrio parahaemolyticus vibrioferrin synthesis gene A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 601
    Illegal SapI.rc site found at 3


Design Notes

Primers for BioBrick construction and cloning:

Forward Primer

5’ – GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA TCT ACG ATG ATT ATC G – 3’

Reverse Primer

5’ – GT TTC TTC CTG CAG CGG CCG CTA CTA GTA TTA TTA TGC TGC CAC CTC CTC GCG AC– 3’

Notes: forward G-C content = 22/50 = 44%; reverse G-C content = 29/55 = 53%



Source

Marine bacterium Vibrio parahaemolyticus

References