Part:BBa_K2797013

High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard

pSB1C3 with a constituitively expressed RFP construct under the control of a medium strength Anderson promoter.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 989
  Illegal suffix found in sequence at 1
  Illegal EcoRI site found at 3052
  Illegal XbaI site found at 3067
  Illegal SpeI site found at 1905
  Illegal PstI site found at 1919
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 989
  Illegal EcoRI site found at 3052
  Illegal NheI site found at 1017
  Illegal NheI site found at 1040
  Illegal SpeI site found at 2
  Illegal SpeI site found at 1905
  Illegal PstI site found at 16
  Illegal PstI site found at 1919
  Illegal NotI site found at 9
  Illegal NotI site found at 995
  Illegal NotI site found at 1912
  Illegal NotI site found at 3058
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 989
  Illegal EcoRI site found at 3052
  Illegal XhoI site found at 2036
  Illegal XhoI site found at 2928
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 989
  Illegal suffix found in sequence at 2
  Illegal EcoRI site found at 3052
  Illegal XbaI site found at 3067
  Illegal SpeI site found at 1905
  Illegal PstI site found at 1919
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 989
  Illegal EcoRI site found at 3052
  Illegal XbaI site found at 1004
  Illegal XbaI site found at 3067
  Illegal SpeI site found at 2
  Illegal SpeI site found at 1905
  Illegal PstI site found at 16
  Illegal PstI site found at 1919
  Illegal AgeI site found at 1627
  Illegal AgeI site found at 1739
  
• 1000
  COMPATIBLE WITH RFC[1000]