Composite

Part:BBa_K2549039

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-09)
Revision as of 10:39, 9 October 2018 by Registry (Talk | contribs)

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VP64-dNLS-ZF21.16

This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64, dNLS and ZF21.16, from N terminal to C terminal. KRAB is a strong transcription repressor. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp, it is cleaved and the transcription activation function cannot be conducted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
//proteindomain/binding
//proteindomain/localization
Parameters
None