Part:BBa_K2539250
ALDH2*2 Basic Part
This gene codes for the mutant form of human mitochondrial acetaldehyde dehydrogenase, ALDH2*2, which metabolizes acetaldehyde at a slower rate compared to wild type ALDH2*2 (Chen et al., 2014).
The ALDH2*2 sequence was based off of ALDH2*1 (NCBI: NM_001204889.1), but modified to remove an internal PstI site. Compared to wild type ALDH2*1, the mutant ALDH2*2 contains a single G to A point mutation, which leads to a change in codon 487, where GAA (coding for glutamic acid) is mutated to AAA (coding for lysine) (Enomoto et al., 1991). The part was confirmed by PCR using the primers VF2 and VR, as well as sequencing by Tri-I Biotech.
PCR Check Results
PCR Check for BBa_K2539250 using the forward and reverse primers VF2 and VR. The expected PCR size of BBa_K2539250 (ALDH2*2 basic part) is around 1800 bp. Bands with the correct size are boxed in yellow.
References
Chen CH, Ferreira JCB, Gross ER, Mochly-Rosen D. (2014). Targeting Aldehyde Dehydrogenase 2: New Therapeutic Opportunities. Physiol Rev. 94(1):1-34.
Enomoto N, Takada A, Date T. (1991). Genotyping of the aldehyde dehydrogenase 2 (ALDH2) gene using the polymerase chain reaction: evidence for single point mutation in the ALDH2 gene of ALDH2-deficiency. Gastroenterol Jpn. 26(4): 440–447.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1247
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 305
Illegal NgoMIV site found at 448
Illegal NgoMIV site found at 961 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 367
Illegal SapI.rc site found at 864
| None |