Coding

Part:BBa_K2812006:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brls   Group: iGEM18_TU-Eindhoven   (2018-10-03)
Revision as of 08:17, 9 October 2018 by MariskaBruls (Talk | contribs) (→‎Source)


Coding sequence for Pyocin S5 with HlyA and His6-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BglII site found at 425
    Illegal BglII site found at 2110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1200


Design Notes

DNA sequence codon optimized for E. coli.


Source

Genomic sequence from Pseudomonas aeruginosa. DNA produced by IDT. (Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.)

References

Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.