Composite

Part:BBa_K2753015

Designed by: WEI KUANGYI   Group: iGEM18_GreatBay_China   (2018-08-29)
Revision as of 08:00, 9 October 2018 by LeoMinghaoLi (Talk | contribs)


pSB1C3-RiboJ-rbs-GPPS-rbs-ObGES

This part consists of a ribozyme (BBa_K1679038), two RBSs, an Abies Grandis geranyl pyrophosphate synthase coding sequence (BBa_K2753002) and an Ocimum basilicum geraniol synthase (BBa_2753003)It metabolizes IPP and DMAPP which are five-carbon blocks produced by either MVA pathway or MEP pathway or in some case both MVA and MEP pathway. IPP and DMAPP are converted to geraniol, a monoterpenoid with a rose-like odour and is a prosperous antimicrobial reagent and gasoline alternative. With addition of more downstream enzymes, geraniol can be changed to a large range of monoterpenes like linalool, actinide and nepetalactol.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2212
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2238


[edit]
Categories
Parameters
None