Coding

Part:BBa_K2739004:Design

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-08)
Revision as of 02:17, 9 October 2018 by Registry (Talk | contribs)

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Phasin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Through sequencing, the previous available Phasin biobrick was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.


Source

The phasin basic part and sequence information were obtained from the Composite part of BBa_K390501.

References