Coding

Part:BBa_K2665011

Designed by: Masahiro Nanbu   Group: iGEM18_Kyoto   (2018-09-25)
Revision as of 02:08, 9 October 2018 by TongYou (Talk | contribs) (→‎Reference)


TDH3-Mangrin-6xHis-CYC

This part coded the mangrin functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast.

Usage and Biology

Mangrin is derived from mangrove and it is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. This part contains only the functional domain(71 amino acids). TDH3 promoter is a constitutive promoter, so this part can express mangrin functional domain constitutively under appropriate condition.

Characterization

For the confirmation of the mangrin function of mangrin, we spotted


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 842
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910


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