Coding
Part:BBa_K2665012:Design
Designed by: You Tong Group: iGEM18_Kyoto (2018-10-08)
TDH3-ZrGPD1-6xHis-CYC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1923
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1454
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1129
Illegal BsaI site found at 1889
Illegal BsaI.rc site found at 1271
Design Notes
We added TDH3 promoter and CYC1 teminator in order to express the ORF, and added His-tag in case of Western Blotting. The ORF sequence is refered from Reference[1].
Source
The ORF of this part is derived from Zygosaccharomyces rouxii.