Coding

Part:BBa_K2683001:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)
Revision as of 22:36, 8 October 2018 by Kristiturton (Talk | contribs) (→‎Design Notes)


Arc Protein Codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 531
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although originally codon optimized for E.coli, sequencing issues lead us to working with genomic DNA.

Source

The original sequence is derived from the Arc protein found in mouse neural cells. The DNA was amplified from the mouse genome and made biobrick compatable by using primer extension PCR and site directed mutagenesis.

References