Composite
proR-phaR

Part:BBa_K2739000

Designed by: Lok In Lo   Group: iGEM18_Edinburgh_OG   (2018-10-08)
Revision as of 22:23, 8 October 2018 by Owen-lo 923 (Talk | contribs)


ProR-PhaR (The phasin autoregulation system with native promoter)

phaR is the core member of the phasin autoregulation system, which contribute to the PHA operon and PHA production. phasin is a low-molecular weight protein that plays a role in PHA granule formation by physically binding to the PHA granule surface and promote the PHA production. The expression of phasin is repressed by an autoregulator phaR, which is able to bind to the region of phasin promoter and its own promoter to regulate the expression of phasin and itself. Otherwise, phaR can also bind to the PHB granules. It is potential to help cells saving energy by curtailing excessive expression of PHA biosynthesis pathways.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 253


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Categories
Parameters
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