Composite

Part:BBa_K2550001:Design

Designed by: Yan Zhang, Janet Standeven, Abby Bell, Megan Hong   Group: iGEM18_Lambert_GA   (2018-10-08)
Revision as of 19:48, 8 October 2018 by Registry (Talk | contribs)

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T7 promoter with a trigger sequence to match the toehold sequence of part BBa_K2550000


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is meant to be used with toehold part. Needs to be used in BL21 cells that have a T7 polymerase.


Source

Collins Paper

References