Part:BBa_K2550001

T7 promoter with a trigger sequence to match the toehold sequence of part BBa_K2550000

T7 Toehold LacZ Trigger is an RNA sequence to be used in conjunction with part BBa_K22550000 to induce LacZ expression for a biosensor system. Toehold Switches are biosensors that can activate gene expression in response to a chosen RNA sequence. It is comprised of a switch and a trigger. The switch is comprised of a hairpin loop that is designed to be complementary to the trigger sequence along with a reporter protein downstream. The RBS and starting sequence is concealed in the toehold switch, preventing the reporter from being expressed. When the trigger RNA sequence is present, it will bind to the complementary sequence in the toehold switch and unbind the hairpin loop allowing the reporter protein to be expressed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 104
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]