Protein_Domain

Part:BBa_K2543008:Design

Designed by: CHUNG-HSUAN HSIAO   Group: iGEM18_Mingdao   (2018-10-08)
Revision as of 18:05, 8 October 2018 by 61XB38 (Talk | contribs) (→‎References)

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Drosophila Toll transmembrane and intracellular domains / pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 793


Design Notes

This part follows the RFC[25] assembly rule with a NgoMIV site in the 5' end.


Source

This part was synthesized by Integrated DNA Technologies, Inc. (IDT) and cloned onto pSB1C3.


References

1. UniProtKB - P08953 (TOLL_DROME)
2. Cell. (1988) The Toll gene of Drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein.
3. Nat Rev Immunol. (2006) Toll-like receptors as molecular switches.
4. J Immunol. (2011) The Drosophila Toll signaling pathway.