Coding

Part:BBa_K2833008

Designed by: Wanji Li   Group: iGEM18_BJRS_China   (2018-10-04)
Revision as of 05:15, 8 October 2018 by BX (Talk | contribs)


J23104+B0032+INP+YFP

Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae，P. flurorescens and several other Gram—negative bacteria.Using its characteristic of forming ice nucleation at relatively warm temperatures(一2~一4‘C)，INP is originally applied in biological refrigeration field.In the bacterial surface display technology，INP is widely used as a functional carrier protein.Compared with other carrier proteins，INP has advantages such as stably expressing foreign proteins and showing the larger molecular weight proteins.INP cell—sur— face display technology has been developed to be a promising approach in many biotechn0109ical fields，such as whole cell bioeatalysts，whole cell bioadsorbence and biodegradation of environmental pollutants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 467
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None