Coding

Part:BBa_K2765030:Design

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-10-07)
Revision as of 04:02, 8 October 2018 by Registry (Talk | contribs)

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1000bp Homologous arm+Ura(△yca1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2416
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1825
    Illegal BsaI.rc site found at 277
    Illegal SapI.rc site found at 559
    Illegal SapI.rc site found at 580
    Illegal SapI.rc site found at 1672


Design Notes

cloned the upstream homologous arm and the downstream homologous arm of yno1 from the genome of CEN.PK2-1C and cloned Leu marker from pESC-Leu.ligased Leu marker and the complete fragment by OE-PCR then transformed  them into CEN.PK2-1C(△yca1).



Source

S. cerevisiae CEN.PK2-1C


References