Coding

Part:BBa_K2683003

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)
Revision as of 03:55, 8 October 2018 by Registry (Talk | contribs)

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MS2 Protein with Polyarginine Peptide

The MS2 protein is a capsid protein originally derived from the MS2 bacteriphage. A surface modification was added whereby a polyarginine peptide sequence of 15 Arginines with a thrombin cleavage site in the linker. Polyarginine tags are known to act as a non-specific Cell penetrating peptide [Schmidt et al., 2010]. This part was codon optimized for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None