Coding

Part:BBa_K2653001:Design

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-07)
Revision as of 01:30, 8 October 2018 by Helen M (Talk | contribs) (→‎Design Notes)

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GFP-nano


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35


Design Notes

The GFP-nano works as a core in our system to degrade the proof of concept target GFP as the tool to bind with it. In the design we link GFP-nano with hIgG1-Fc, so that it can work as the bridge between target GFP and Trim21, and finally help form a ternary complex of GFP, GFP-nano and Trim21. Only after this the degradation could successfully be processed.

Source

The sequence of GFP-nano we use came from a patent.

References