Coding

Part:BBa_K2653001

Designed by: Jiaxin Ma   Group: iGEM18_NUDT_CHINA   (2018-10-07)
Revision as of 01:11, 8 October 2018 by Registry (Talk | contribs)

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GFP-nano

The GFP-nano is designed to bind with both Trim21 and the antigen GFP to prove that the P.R.PREDATOR really works. It belongs to the single-chain variable fragment(scFv) family, which has the ability to bind with GFP, but is much smaller than the common antibody of GFP(only 364 bps). In the project GFP-nano successfully bound with target GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35


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Categories
Parameters
None