Tag
His-Tag

Part:BBa_K2834002

Designed by: Viana Isabel Pérez Domínguez   Group: iGEM18_Tec-Chihuahua   (2018-10-07)


6x His-Tag

Defensin 2 has selective activity against Gram-positive bacteria. This peptide disrupts the permeability barrier of the cytoplasmic membrane by forming channels, resulting in a loss of cytoplasmic K+, a partial depolarization of the inner membrane, a decrease in cytoplasmic ATP, and inhibition of respiration processes.

Cociancich, S., Ghazi, A., Hetru, C., Hoffman, J. A., & Letellier, L. (1993). Insect Defensin, an Inducible Antibacterial Peptide, Forms Voltage-dependent Channels in Micrococcus luteus. The Journal of Biological Chemistry, 268(26), 19239-19245

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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