Coding

Part:BBa_K2871003

Designed by: Nattawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-10-07)
Revision as of 17:53, 7 October 2018 by Nleelahakorn (Talk | contribs)

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CesF T3SS substrate chaperone

CesF and CesT are chaperone proteins that facilitate effector protein secretion in E. coli type-III secretion system. CesF has been proven to be necessary for secretion of EspF, while CesT is essential for Tir and Map effector protein secretion. We try to incorporate those two chaperones into our system because it was omitted in the engineered E. coli T3SS system we are using.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 21
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 52


[edit]
Categories
Parameters
None