Part:BBa_K2570010:Design

Production of 2-PE: Aro10+PAR+TyrB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 2242
  Illegal NotI site found at 2605
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4044
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Glutamate dehydrogenase plays a key role in 2-PE biosynthesis.

Source

The phenyl pyruvate decarboxylase from S. cerevisiae (Aro10)

https://www.ncbi.nlm.nih.gov/protein/NP_010668.3?report=genbank&log$=protalign&blast_rank=1&RID=V0V04K3B015

the phenyla-cetaldehyde reductase from the rose (PAR)

https://www.ncbi.nlm.nih.gov/protein/BAG13450.2?report=genbank&log$=protalign&blast_rank=1&RID=V0NV9P35014

The aromatic transaminase from E. coli (TyrB)

https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014

References

Wang, P., Yang, X., Lin, B., Huang, J., & Tao, Y. (2017). Cofactor self-sufficient whole-cell biocatalysts for the production of 2-phenylethanol. Metabolic Engineering, 44(August), 143–149. https://doi.org/10.1016/j.ymben.2017.09.013