Part:BBa_K2568017
Virus receptor TfR with Kozak sequence
The way how viruses enter into cells is mainly mediated by host receptors. We have established an association between virus Baltimore subtyping and host receptors, and found 4 internalization receptors and 2 attachment molecules that are necessary to mediate the entry of Baltimore types I to V. Our chassis cell MDBK has most internalization receptors and adhesion molecules, but lacks Nectin 4 and TfR to enable the production of most of the commonly seen animal viruses. TfR is one of the receptors that mediates the entry of canine parvovirus (CPV). As a kind of cattle cell, MDBK cannot be naturally infected by canine viruses, but can become sensitive to CPV infection if TfR was functionally expressed on cell surface. Therefore, we are motivated to construct the TfR biobrick to achieve the goal of cultivating multiple viruses in MDBK cells..
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 721
- 1000COMPATIBLE WITH RFC[1000]
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