Composite

Part:BBa_K2807014

Designed by: Ravichandran Divyapoorani   Group: iGEM18_NUS_Singapore-Sci   (2018-10-07)
Revision as of 09:54, 7 October 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


eGFP-GLB1 WT

This eGFP-GLB1 part is a GFP fusion protein linked to the human GLB1 gene. GLB1 encodes a human lysosomal acid beta galactosidase, an enzyme that is responsible for the cleavage of terminal β-linked galactose residues from glycoproteins, sphingolipids, keratan sulfate, and other glycoconjugates. The eGFP-GLB1 fusion protein was designed as an alternative base editing reporter for potential application in a disease model in the future. In our project, it served the purpose of detecting whether our base editing system, rAPOBEC1-XTEN-dPspCas13b along with a guide RNA, is able to make the specific base changes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 764
    Illegal BglII site found at 797
    Illegal BamHI site found at 828
    Illegal BamHI site found at 2185
    Illegal BamHI site found at 2521
    Illegal XhoI site found at 768
    Illegal XhoI site found at 801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None