Part:BBa_K2794001

CD63 Varient 1 (Homo Sapiens)

CD63 Varient 1 (Homo Sapiens)
Function	Exosome Membrane Anchoring
Use in	Mammalian cells
RFC standard	RFC 10 compatible
Backbone	pSB1C3
Submitted by	[http://2018.igem.org/Team:SHSU_China SHSU_China]

Overview

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.

CD63 (varient 1) passes through the membrane 4 times. The N- and C- terminus is both located inside the membrane. The protein has two extracellular cringle (EC1, EC2) and one intracellular loop (IL), with EC2 significantly longer than EC1 and carries main cellular functions.

CD63 is recently researched and used in the field of extracellular vesicle (EVs) research. Due to its strong expression on EVs, it is used in exosome identification, extraction and targeted therapy. A protein sequence can be attached to either the N- or C- terminus so that a protein cargo can be loaded into exosomes using CD63. ()

Experience

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]