Composite

Part:BBa_K2836002

Designed by: Chang Wei-Tse   Group: iGEM18_SHSID_China   (2018-10-07)
Revision as of 08:01, 7 October 2018 by Registry (Talk | contribs)

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luxCDABEG-luxG

This bacterial lux operon (luxCDABEG)encodes for the endogenous reactants needed in a light-emitting chemical reaction in the bioluminescent bacteria Aliivibrio fischeri. The luxA and luxB genes code for the alpha and beta subunits of the bacterial luciferase, luxC, luxD, and luxE genes encode for the r,s, and t polypeptides of the fatty-acid reductase complex that synthesizes and recycles the aldehyde substrate for luciferase, and luxG codes for the flavin reductase needed in regenerating the reduced flavin mononucleotide used in the reaction. As the flavin reductase has been shown to increase light production in bacteria, the 2018 SHSID_China iGEM team has synthesized and attached another luxG gene from the same species (but different strain) onto the original lux operon in hopes of increasing light output.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 4171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3169
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5558
    Illegal BsaI.rc site found at 2567
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5883


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Parameters
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