Part:BBa_K2568010:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out. BBa_K1493801 contains two TetR operator sites at the core and proximal positions (middle and right parts of the promoter).We transduced the recombinant plasmid containing the target promoter and an rfp reporter gene into E.coli. In this experiment, we chose BioTek Synergy multifunctional enzyme mark to measure the fluorescence intensity and OD450, PBS was used as the blank control. According to the optimum detection wavelength of RFP, the parameters for detecting fluorescence intensity were: excitation wavelength of 584nm and emission wavelength of 607nm. The fluorescence / OD450 was used to measure the RFP expression level of bacteria.

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