Coding
surCD19

Part:BBa_K2549001

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 05:44, 7 October 2018 by Wave (Talk | contribs) (→‎Usage and Biology)


suface-expressed CD19

Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies[1]. CD8α peptide guides synthesized fusion protein to pass the translocon[2] into the endoplasmic reticulum[3], and the fusion protein will be later sugar modified in Golgi[4], presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane. It was used as the antigen for Part:BBa_K2549005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757
    Illegal SapI site found at 273
    Illegal SapI.rc site found at 91

Biology

Clinical significance of CD19
Reported data from Royal et al 2016

References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
  2. ↑ https://en.wikipedia.org/wiki/Translocon
  3. ↑ https://en.wikipedia.org/wiki/Endoplasmic_reticulum
  4. ↑ https://en.wikipedia.org/wiki/Golgi_apparatus
[edit]
Categories
//collections/immune_regulation/receptors
Parameters
protein