Composite

Part:BBa_K2888009

Designed by: SBS_SH_112144   Group: iGEM18_SBS_SH_112144   (2018-10-07)
Revision as of 05:21, 7 October 2018 by Registry (Talk | contribs)

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fused mlrA gene

This mlrA gene is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1. In our experiment, we intend to deal with Microcystin-LR, the most common type of microcystin toxin produced in cyanobacteria. The mlrA gene is responsible for hydrolytic cleavage and linearization of cyclic structure of MCLR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal AgeI site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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