Composite
Part:BBa_K2888008
Designed by: SBS_SH_112144 Group: iGEM18_SBS_SH_112144 (2018-10-07)
fused chimerical gene with 6x His tag (lysozyme and mlrA)
We attempt to link the MC-degrading enzyme and the cyanobacteria phage-originated lysozyme to produce a protein that possesses the function of both enzymes by genetically modifying E.coli . We also make a solution with the Bugbuster and the putative functional enzymes to perform cyanobacteria lysis and MC degradation simultaneously. Furthermore, we purposely add 6x histag into our sequence in order to improve the efficacy and efficiency of expression and purification; it will also strengthen effects of binding to Ni-NTA column, making it possible to immobilize proteins and lyse cyanobacteria repeatedly in our prototype device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1349
Illegal AgeI site found at 884 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |