Composite
NG8-1

Part:BBa_K2623028

Designed by: Niangui Cai   Group: iGEM18_XMU-China   (2018-10-06)
Revision as of 04:59, 7 October 2018 by Registry (Talk | contribs)

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A composite part linked by SpyTag-OmpA and SpyCatcher-L7Ae-mRFP1


Under the induction of IPTG, this part can code SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1965
    Illegal NheI site found at 1988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 3122
    Illegal AgeI site found at 3234
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None