Part:BBa_K2549001

suface-expressed CD19

Surface-expressed CD19 (surCD19) is built by joining CD8α signal peptide, CD19 extracellular domain and the transmembrane region of PDGFRβ (from N terminal to C terminal). Additional HA tag on its N terminal and a Myc tag on its C terminal to facilitate detection by antibodies^[1]. CD8α peptide guides synthesized fusion protein to pass the translocon^[2] into the endoplasmic reticulum^[3], and the fusion protein will be later sugar modified in Golgi^[4], presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 588
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 757
Illegal SapI site found at 273
Illegal SapI.rc site found at 91

Usage and Biology

Cellular localization

Function as the antigen for Part:BBa_K2549005

References

↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
↑ https://en.wikipedia.org/wiki/Translocon
↑ https://en.wikipedia.org/wiki/Endoplasmic_reticulum
↑ https://en.wikipedia.org/wiki/Golgi_apparatus

[1] Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012

[2] ttps://en.wikipedia.org/wiki/Translocon

[3] ttps://en.wikipedia.org/wiki/Endoplasmic_reticulum

[4] ttps://en.wikipedia.org/wiki/Golgi_apparatus

[1]

[2]

[3]

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