Composite
crRNA-rrnB

Part:BBa_K2627004:Design

Designed by: Zhaoqin Zhang   Group: iGEM18_SDU-CHINA   (2018-10-06)
Revision as of 12:11, 6 October 2018 by Registry (Talk | contribs)

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crRNA targeting GltA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 314


Design Notes

We managed to delete the restriction site for EcoRI through Gibson Assembly.


Source

Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1).

References