Composite

Part:BBa_K2789027

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)
Revision as of 11:56, 6 October 2018 by Registry (Talk | contribs)

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Plac+RBS+lectin-FimH+terminator

This part is the standard expression pathway of K2789026： lectin-FimH， this part can also display the lectin to the surface of the bacteria. Lectin is a protein that originally from Green algae. It has positive charge thus can bind with EPS(exopolysubstance),which is secreted by algae. In our project, we wanted our bacteria to connect with the algae to strengthen the symbiotic biofilm. Thus we wanted to make it expressed on the surface of bacteria. Fusing lectin with FimH is another way to display it on bacteria surface. FimH is a part of pili，some teams had identified that make fused protein with this protein can make the target protein expressed on the surface.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 523
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 523
    Illegal AgeI site found at 187
    Illegal AgeI site found at 325
    Illegal AgeI site found at 426
    Illegal AgeI site found at 681
    Illegal AgeI site found at 823
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None