Composite

Part:BBa_K2789025

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)
Revision as of 11:34, 6 October 2018 by Registry (Talk | contribs)

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Plac+RBS+lectin-OmpA+terminator

This a a standard expression pathway of K2789024. Lectin is a protein that originally from Green algae. It has positive charge thus can bind with EPS(exopolysubstance),which is secreted by algae. In our project, we wanted our bacteria to connect with the algae to strengthen the symbiotic biofilm. Thus we wanted to make it expressed on the surface of bacteria. We used surface display technology to do that job. The coding sequence of OmpA and lectin had been fused and thus can express the lectin on the surface of the bacteria. Then make it connect with the EPS of algae! You can use this part to bind other agents with negative charges.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 388
    Illegal AgeI site found at 577
    Illegal AgeI site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None