Coding

Part:BBa_K2788002:Design

Designed by: chen liang   Group: iGEM18_SZU-China   (2018-10-05)
Revision as of 10:03, 6 October 2018 by M Orz (Talk | contribs) (→‎Source)


Encoded by the gene MCL1 from Metarhizium robertsii ARSEF 23


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1688
    Illegal NgoMIV site found at 1739
    Illegal NgoMIV site found at 1864
    Illegal AgeI site found at 1670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1106
    Illegal SapI site found at 1882
    Illegal SapI site found at 1901


Design Notes

The studies on this protein are slim,there is no prepared antibody on sale.So if you want to study on its expression,for example,you want to do Western Blot and other itechnique based on antigen-antibody reaction,you'd better customize antibody in advance.Besides,preparation of histidine label fusion protein is an alternative proposal.


Source

/organism="Metarhizium robertsii"
/mol_type="mRNA"
/strain="ARSEF 23"

References

Hu X, Xiao G, Zheng P, Shang Y, Su Y, Zhang X, Liu X, Zhan S, St Leger RJ, Wang C.Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation.Proc Natl Acad Sci U S A. 2014 Nov 25; 111(47):16796-801. Epub 2014 Nov 3. 

Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci USA. 2006;103(17):6647–6652.