Signalling

Part:BBa_K2789009:Design

Designed by: Du liaoqi   Group: iGEM18_WHU-China   (2018-10-05)
Revision as of 03:57, 6 October 2018 by Registry (Talk | contribs)

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optimized Ccas from BBa_K68102


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 715
    Illegal AgeI site found at 1720
    Illegal AgeI site found at 1915
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have optimized the code in the Ccas and we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.


Source

initial Ccas from BBa_K68102

References