Composite

Part:BBa_K2804005

Designed by: Byron Francisco Mosquera   Group: iGEM18_Ecuador   (2018-10-05)
Revision as of 19:47, 5 October 2018 by Registry (Talk | contribs)

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CBD cipA fused to sfGFP under the control of LacI promoter

The superfolder green fluorescent protein (sfGFP) is fused to the cellulose binding domain (CBD) of the scaffolding protein of Clostridium thermocellum. This part is flanked by preffix and suffix in RFC10.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None