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Part:BBa_K2609013

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-24)
Revision as of 18:47, 5 October 2018 by BhaskarK (Talk | contribs)


gp37 under T7 expression system - Mod II

Coding sequence of the long tail fiber protein from T4 bacteriophage under the T7 expression system. The protein is fused to a 6xHis tag at the N-term through a enterokinase tag for easy purification. The sequence has been modified in-silico for better binding to phosphoethanolamine near the receptor binding end of the protein. This is the first such modification in our series of four modifications.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1239
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 507
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.
[3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.
[4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292.

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