Composite

Part:BBa_K2804001

Designed by: Byron Francisco Mosquera   Group: iGEM18_Ecuador   (2018-10-05)
Revision as of 18:46, 5 October 2018 by Registry (Talk | contribs)

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CBD cipA fused to BMP2 under the control of LacI promoter

The human bone morphogenetic protein 2 (BMP2) is fused to the C-terminus of the cellulose binding domain (CBD) of the scaffolding protein cipA of Clostridium thermocellum. This part is flanked by preffix and suffix in RFC10.

Because of a BMP2 C-terminal domain provides the osteoginic activity to the protein (Schmoekel et al., 2005), we have decided to fuse it N-terminally to the CBD cipA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 1106
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None