Coding
TEVp
Part:BBa_K2549013:Design
Designed by: Rongrong Du Group: iGEM18_Fudan (2018-10-05)
TEV protease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 316
Design Notes
The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient catalyst[1].
Source
IDT(gBlock)