Coding
TEVp

Part:BBa_K2549013

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-05)
Revision as of 13:41, 5 October 2018 by Registry (Talk | contribs)

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TEV protease

This part is a mutant TEV(tobacco etch virus) protease. The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient catalyst[1]. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gate. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316


[edit]
Categories
//cds/enzyme/protease
//function/degradation
Parameters
None