Part:BBa_R0085

T7 Consensus Promoter Sequence

The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.

Usage and Biology

This promoter is the consensus T7 promoter running from -17 to +6.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Improvement of R0085 by ZJU-CHINA 2018 Teams

The ZJU-CHINA 2018 Teams fulfilled the improvement of R0085 by inducing some mutation in the sequence. A series of promoters with increased expression strength are constructed. The result can be seen by clicking the link below.

<a href=https://parts.igem.org/Part:BBa_K2721000>BBa_K2721000</a>

<a href=https://parts.igem.org/Part:BBa_K2721001>BBa_K2721001</a>

<a href=https://parts.igem.org/Part:BBa_K2721002>BBa_K2721002</a>

<a href=https://parts.igem.org/Part:BBa_K2721003>BBa_K2721003</a>