Composite

Part:BBa_K2558206:Design

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
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CRISPRi device with Ptac expressed gRNA and Anderson strong (J23100)- expressed lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 310
    Illegal NheI site found at 333
    Illegal NheI site found at 1466
    Illegal NheI site found at 4626
    Illegal NheI site found at 4649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5830
    Illegal BamHI site found at 3745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

[1] Gilbert LA et al. Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell. 2014 Oct 23;159(3):647-61. [2] iGEM parts: PHIF repressible promotor (https://parts.igem.org/Part:BBa_K1725000)

References