Composite

Part:BBa_K2558205

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
Revision as of 11:27, 5 October 2018 by Registry (Talk | contribs)

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Ptac test device with Anderson weak-expressed lacI

Tac promotor is a hybrid between top and lacUV5 promoters. Tac promotor is a kind of strong E. coli promotors and is IPTG inducible. This device contains constitutively (BBa_J23114, Anderson weak promotor) expressed lacI protein and Ptac controlled sfGFP reporter. We designed this device along with K2558203 and K2558204, which are the same except for the strength of the constitutive lacI expression. We aim to specify the relation between the level of lacI production and the quality of IPTG induced expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1073
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 176


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