DNA

Part:BBa_K2711000:Design

Designed by: Lisa Fröhlich   Group: iGEM18_UiOslo_Norway   (2018-09-23)
Revision as of 11:05, 5 October 2018 by Markushovd (Talk | contribs)


Glucanase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 958
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence had to be codon-optimized due to difficulty of synthesizing.


Source

Cellulosimicrobium cellulans

References

1. Deepak Mudgil, in Dietary Fiber for the Prevention of Cardiovascular Disease, 2017

2. Salazar et al., 2001. Overproduction, Purification, and Characterization of β-1,3- Glucanase Type II in Escherichia coli. Protein Expression and Purification 23, 219–225.

3. Oda et al., 2017. Structural and thermodynamic characterization of endo-1,3-β-glucanase: Insights into the substrate recognition mechanism. BBA - Proteins and Proteomics 1866 (2018) 415–425.