Coding
Part:BBa_K2570002:Design
Designed by: Yuxian Chen Group: iGEM18_FJNU-China (2018-09-26)
TyrB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1054
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Site-directed mutation site
TyrB(Pst1:CTGCAG—CTGCAA)
sense primer: GGTGGCTGCAATGCTGAATGACG
Anti-sense primer: TCAGCATTGCAGCCACCACCTG
Source
Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.