DNA
Part:BBa_P10101:Experience
Designed by: Traci Haddock-Angelli Group: Plants (2016-05-25)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_P10101
The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
File:Https://static.igem.org/mediawiki/parts/3/34/35S+RTBV GUS NosT results.png
Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.
File:Https://static.igem.org/mediawiki/2018/1/19/T--Cardiff Wales--mCherryPromoterAssayQuantified.png
Figure 2) The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too.
User Reviews
UNIQb2a0e858e7a9a4ae-partinfo-00000000-QINU UNIQb2a0e858e7a9a4ae-partinfo-00000001-QINU