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Applications of BBa_P10101

The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.

File:Https://static.igem.org/mediawiki/parts/3/34/35S+RTBV GUS NosT results.png

Figure 1) The GUS staining with the upper row of leaves is 35S, and is much stronger than the lower leaves which are using the RTBV promoter instead.

File:Https://static.igem.org/mediawiki/2018/1/19/T--Cardiff Wales--mCherryPromoterAssayQuantified.png

Figure 2) The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue in the table below it too.

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Status: 500 Content-type: text/html

Software error:

Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 248, near "range;"
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 249, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 249, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 259, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 280, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 286.
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 312, near "}"
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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